医学科研型研究生常用实验技术与方法
医学科研型研究生常用实验技术与方法
本着常用、基本的编写原则,让医学研究生掌握科研实验的基本功,顺利完成学业
内容简介本书是在充分了解医学科研型研究生的实验需求基础上,由科研技术人...
本着常用、基本的编写原则,让医学研究生掌握科研实验的基本功,顺利完成学业
本书是在充分了解医学科研型研究生的实验需求基础上,由科研技术人员发挥专业特长所编写。分为三大部分,共22章。部分为实验室通识,包括现代生物医学基础研究的模式和方法、实验室生物安全、科研实验记录书写要求与规范和实验用水、溶液与缓冲液;第二部分为常用实验技术,包括细胞培养常用实验技术、常用实验动物基本知识与操作、蛋白质含量测定和酶联免疫吸附试验、PCR的原理及应用、蛋白质免疫印迹技术原理与操作、免疫细胞与组织化学技术、流式细胞术、分子克隆技术、基因修饰小鼠技术原理、中药有效成分提取分离及干燥技术等;第三部分为专题研究方法,包括细胞凋亡检测常用方法、细胞自噬检测方法、外泌体研究方法、骨代谢生物学研究方法、高效液相色谱在中药研究中的应用、中药网络药理学及常用数据库简介、组学研究方法、动物与细胞实验的系统评价与Meta分析等。
戴薇薇,博士,中西医结合生物化学专业,上海中医药大学副教授,龙华医院科技中心实验室主任,从事实验室管理与研究生科研实验教学工作,中医药延缓衰老与防治老年病机制研究。
一·现代生物医学研究的模式002
二·生物医学基础研究的三种实验系统003
三·围绕“分子生物学中心法则”展开的实验方法体系004
一·实验室生物安全概念006
二·实验室安全制度管理007
三·实验室化学废弃物的处理010
四·实验室意外事故应急预案012
一·实验记录的意义017
二·实验记录的原则017
三·实验记录书写规范要求019
一·实验室用水020
二·溶液022
三·溶液的浓度023
医学科研型研究生需要接受基础技术的系统培训,才能胜任今后的科研工作。有鉴于此,在充分了解学生的实验需求基础上,科研技术人员发挥专业特长,编写了《医学科研型研究生常用实验技术与方法》。本书分为实验室通识、常用实验技术和专题研究方法三大部分,共22章,可供需要开展科研实验的医学硕士生、博士生和从事基础生物医学研究的教师、医师,以及学有所长的本科生学习。
本书以“常用、基本”为原则,所述常用技术相当于科研实验的基本功,对于医学研究生而言,掌握这些常用技术可基本够用;学会这些技术,大部分医学科研实验能顺利完成。但还有许多技术,如电生理技术,未收入本书中,实验者在应用前需要有针对性地学习。
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• Domestic Shipping: Orders within the U.S. are shipped via USPS or FedEx, depending on the origin of the product. The average transit time is 3-7 business days.
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• Tracking Information: Every order is trackable. You will receive a tracking number once your order has been shipped. Products may be shipped from various global fulfillment centers.
Shipping Delays:
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Exclusions: Gift cards, packaging, taxes, and prior purchases do not count toward the minimum purchase requirement for free shipping. This offer is valid only for shipments to U.S. addresses, including Puerto Rico.
Delivery Details:
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Estimates: Standard shipping within the US typically takes 3-7 business days. These are estimates and not guarantees.
-
Shipping Restrictions: We ship to all 50 states, Washington, DC, U.S. territories, and APO/FPO/DPO addresses. Shipping options vary based on the delivery address.
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Remote Areas: Shipments to remote areas may incur additional charges or require pickup from a nearby shipping partner’s location.
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You will receive a shipping confirmation email with a tracking number as soon as your order is dispatched. If you do not receive this email immediately, please be assured that your items will arrive within the estimated delivery window provided at checkout.
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If you need to cancel or modify your order, please contact our customer support immediately.
Issues with Delivery:
If your order shows as delivered but you have not received it, please contact the shipping carrier directly to resolve the issue. For persistent problems, contact our customer service at cs@everymarket.com.
Customer Support:
Our team is available 24/7 to assist you with any questions or concerns regarding your order. We are committed to ensuring a smooth shopping experience.
Return & Refund Policy Overview
Please review our return and refund policies below to ensure a smooth transaction process.
Return Policy
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Duration: You have 30 days from receiving your item to initiate a return.
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Condition: Items must be unworn, unwashed, with original tags and packaging intact.
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Shipping Costs: Customers are responsible for return shipping costs.
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Packaging: Ensure returned items are well-packaged to avoid damage during transit.
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Tracking: Use a trackable and insured shipping method as we are not liable for items lost or damaged on return.
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Initiating a Return: Contact us at cs@everymarket.com to start your return. We will provide a return shipping label and instructions upon approval. Returns without prior approval will not be accepted.
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Method: Returns must be sent back by mail to the address provided in the return instructions.
Return Label
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Defective Products: Return labels are provided for defective items.
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Non-Defective Returns: Customers are responsible for obtaining their return shipping label.
Product Conditions for Return
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Eligible Products: Returns are only accepted for items in new condition.
Nonreturnable Items
Certain items are not eligible for return:
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Electronic devices after 30 days (e.g., computers, laptops, Kindles)
-
Gift cards, prepaid game cards
-
Perishable goods, live insects, some jewelry, some health and personal care items
-
Customized or personalized products
-
Items with special shipping restrictions
Refund Policy
-
Window: Eligible products may be returned within 30 days of delivery for a refund.
-
Refund Method: Refunds are processed to the original payment method or as store credit for items purchased with gift cards.
-
Processing Time: Refunds are processed within 3-5 business days after we receive the return; please allow additional time for shipping and bank processing.
Claims
Inspect your order upon arrival and report any defects, damages, or incorrect items immediately to allow us to address the issue. For claims, contact our support team with details of the issue.
Exchange Policy
For the quickest service, return your original item and place a new order for the desired product once your return is accepted.
Return Address
EveryMarket Customer Service 2101 E Terra Ln, O'Fallon, MO 63366
Customer Support
Available 24/7 for any questions or assistance needed:
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Phone: +1 636-312-5925
-
Email: cs@everymarket.com
本着常用、基本的编写原则,让医学研究生掌握科研实验的基本功,顺利完成学业
本书是在充分了解医学科研型研究生的实验需求基础上,由科研技术人员发挥专业特长所编写。分为三大部分,共22章。部分为实验室通识,包括现代生物医学基础研究的模式和方法、实验室生物安全、科研实验记录书写要求与规范和实验用水、溶液与缓冲液;第二部分为常用实验技术,包括细胞培养常用实验技术、常用实验动物基本知识与操作、蛋白质含量测定和酶联免疫吸附试验、PCR的原理及应用、蛋白质免疫印迹技术原理与操作、免疫细胞与组织化学技术、流式细胞术、分子克隆技术、基因修饰小鼠技术原理、中药有效成分提取分离及干燥技术等;第三部分为专题研究方法,包括细胞凋亡检测常用方法、细胞自噬检测方法、外泌体研究方法、骨代谢生物学研究方法、高效液相色谱在中药研究中的应用、中药网络药理学及常用数据库简介、组学研究方法、动物与细胞实验的系统评价与Meta分析等。
戴薇薇,博士,中西医结合生物化学专业,上海中医药大学副教授,龙华医院科技中心实验室主任,从事实验室管理与研究生科研实验教学工作,中医药延缓衰老与防治老年病机制研究。
一·现代生物医学研究的模式002
二·生物医学基础研究的三种实验系统003
三·围绕“分子生物学中心法则”展开的实验方法体系004
一·实验室生物安全概念006
二·实验室安全制度管理007
三·实验室化学废弃物的处理010
四·实验室意外事故应急预案012
一·实验记录的意义017
二·实验记录的原则017
三·实验记录书写规范要求019
一·实验室用水020
二·溶液022
三·溶液的浓度023
医学科研型研究生需要接受基础技术的系统培训,才能胜任今后的科研工作。有鉴于此,在充分了解学生的实验需求基础上,科研技术人员发挥专业特长,编写了《医学科研型研究生常用实验技术与方法》。本书分为实验室通识、常用实验技术和专题研究方法三大部分,共22章,可供需要开展科研实验的医学硕士生、博士生和从事基础生物医学研究的教师、医师,以及学有所长的本科生学习。
本书以“常用、基本”为原则,所述常用技术相当于科研实验的基本功,对于医学研究生而言,掌握这些常用技术可基本够用;学会这些技术,大部分医学科研实验能顺利完成。但还有许多技术,如电生理技术,未收入本书中,实验者在应用前需要有针对性地学习。
Shipping Overview:
• Shipping: Standard Domestic Shipping within the United States charges USD 4.99. Standard International Shipping from United Kingdom, Germany and Japan to the United States charges 14.99.
• Order Processing: Please allow 1-2 business days for order processing and preparation before shipment.
• Domestic Shipping: Orders within the U.S. are shipped via USPS or FedEx, depending on the origin of the product. The average transit time is 3-7 business days.
• International Shipping: Currently, we only ship within the USA.
• Tracking Information: Every order is trackable. You will receive a tracking number once your order has been shipped. Products may be shipped from various global fulfillment centers.
Shipping Delays:
Please note that shipping times may vary due to factors beyond our control, such as weather conditions, natural disasters, or peak holiday periods. While we strive to ensure timely delivery, the exact arrival time cannot be guaranteed and is managed by the shipping carrier.
Shipping Options:
-
Standard Delivery: Most orders are shipped within 3-7 business days. Larger items may utilize LTL shipping for safe handling.
-
Handling Time: We handle shipments on business days (Monday - Friday), with a preparation time of 1-2 days.
-
Additional Charges: Some items require additional shipping charges due to their size, weight, or special handling. These charges are specified on the product pages and are not eligible for shipping discounts.
-
Exclusions: Gift cards, packaging, taxes, and prior purchases do not count toward the minimum purchase requirement for free shipping. This offer is valid only for shipments to U.S. addresses, including Puerto Rico.
Delivery Details:
-
Estimates: Standard shipping within the US typically takes 3-7 business days. These are estimates and not guarantees.
-
Shipping Restrictions: We ship to all 50 states, Washington, DC, U.S. territories, and APO/FPO/DPO addresses. Shipping options vary based on the delivery address.
-
Remote Areas: Shipments to remote areas may incur additional charges or require pickup from a nearby shipping partner’s location.
Shipping Confirmation:
You will receive a shipping confirmation email with a tracking number as soon as your order is dispatched. If you do not receive this email immediately, please be assured that your items will arrive within the estimated delivery window provided at checkout.
Order Modifications:
If you need to cancel or modify your order, please contact our customer support immediately.
Issues with Delivery:
If your order shows as delivered but you have not received it, please contact the shipping carrier directly to resolve the issue. For persistent problems, contact our customer service at cs@everymarket.com.
Customer Support:
Our team is available 24/7 to assist you with any questions or concerns regarding your order. We are committed to ensuring a smooth shopping experience.
Return & Refund Policy Overview
Please review our return and refund policies below to ensure a smooth transaction process.
Return Policy
-
Duration: You have 30 days from receiving your item to initiate a return.
-
Condition: Items must be unworn, unwashed, with original tags and packaging intact.
-
Shipping Costs: Customers are responsible for return shipping costs.
-
Packaging: Ensure returned items are well-packaged to avoid damage during transit.
-
Tracking: Use a trackable and insured shipping method as we are not liable for items lost or damaged on return.
-
Initiating a Return: Contact us at cs@everymarket.com to start your return. We will provide a return shipping label and instructions upon approval. Returns without prior approval will not be accepted.
How to Return
-
Method: Returns must be sent back by mail to the address provided in the return instructions.
Return Label
-
Defective Products: Return labels are provided for defective items.
-
Non-Defective Returns: Customers are responsible for obtaining their return shipping label.
Product Conditions for Return
-
Eligible Products: Returns are only accepted for items in new condition.
Nonreturnable Items
Certain items are not eligible for return:
-
Electronic devices after 30 days (e.g., computers, laptops, Kindles)
-
Gift cards, prepaid game cards
-
Perishable goods, live insects, some jewelry, some health and personal care items
-
Customized or personalized products
-
Items with special shipping restrictions
Refund Policy
-
Window: Eligible products may be returned within 30 days of delivery for a refund.
-
Refund Method: Refunds are processed to the original payment method or as store credit for items purchased with gift cards.
-
Processing Time: Refunds are processed within 3-5 business days after we receive the return; please allow additional time for shipping and bank processing.
Claims
Inspect your order upon arrival and report any defects, damages, or incorrect items immediately to allow us to address the issue. For claims, contact our support team with details of the issue.
Exchange Policy
For the quickest service, return your original item and place a new order for the desired product once your return is accepted.
Return Address
EveryMarket Customer Service 2101 E Terra Ln, O'Fallon, MO 63366
Customer Support
Available 24/7 for any questions or assistance needed:
-
Phone: +1 636-312-5925
-
Email: cs@everymarket.com
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