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医学形态学染色技术理论与应用
$41.63
本书理论与实践结合,内容系统,可操作性和实用性强,是一本较好的医学形态学染色技术工具书。
本书是为适应医学形态学染色技术广泛应用于实验标...
本书是为适应医学形态学染色技术广泛应用于实验标本制作和人体数字切片库建立的需要而编写的。书中涵盖了医学形态学的相关理论,并以生物组织制片与染色技术为着眼点和重心,对涉及的必要理论知识进行了较系统的讲解。详细介绍了各种组织染色与组织切片制作的操作步骤、注意事项和适用范围等,对一些技术难点和容易发生问题的地方做了提醒,并给出了一定的解决办法。书中附有彩色图片。本书理论与实践紧密结合,内容系统、全面、丰富,可操作性和实用性强,是一本较好的医学形态学染色技术工具书,可供广大医学院校教师、实验技术员、科研人员、选修医学形态学实验课程的本科生与研究生阅读参考。。
龚林,毕业于泸州医学院,副研究员。中国细胞学会会员,中国解剖学会会员。发辫论文40余篇,获、省级教学奖8项,荣获国家发明专利2项。
第1 章 概述 1一、医学形态学染色技术的内容与意义 1二、医学形态学染色技术的发展 1三、常用的染色方法 5四、细胞核染色剂(碱性染料) 6五、苏木精的种类、配法及其应用 9六、细胞质染色剂(酸性染料) 10七、苏木精- 伊红液的配法及染色法 12八、苏木精- 伊红染色法(HE 染色法) 17九、全自动染色机的应用 19十、常用特殊染色技术 19十一、特殊染色方法的应用 21第2 章 上皮组织 23一、动物的选择 23二、反应原理 24三、染色方法 24第3 章 结缔组织 29一、疏松结缔组织 29二、脂肪组织 31三、疏松结缔组织铺片制作 32四、疏松结缔组织铺片制作具体方法 34五、弹性纤维染色方法的应用 40六、胶原纤维染色方法的应用 47七、网状纤维染色方法的应用 52八、肥大细胞 592医学形态学染色技术理论与应用九、浆细胞 62十、脂肪 63第4 章 血液 68一、血液的组成成分与红细胞的形态特点 68二、网织红细胞 69三、罗氏多色性染剂 70四、血涂片染料类型与染液的制备 70五、血涂片具体制作方法 73六、网织红细胞染色方法 77七、淋巴及骨髓 78笫5 章 软骨和骨 80一、软骨 80二、骨 87第6 章 肌组织 96一、肌组织的类型 96二、肌组织取材 97三、肌肉组织染色法 98第7 章 神经组织 107一、神经元及神经纤维的结构 107二、神经元胞质内特征性结构 108三、神经胶质细胞 109四、神经末梢 109五、神经组织染色技术的起源与发展 110六、金属浸染法在神经组织中的作用机制 112七、调色与固定(定影)在神经组织中的作用机制 113八、神经组织染色方法 114第8 章 神经系统 152一、大脑 152二、小脑 153三、脊髓 1533目录四、神经节 153五、神经系统取材 154六、脑取出后灌注固定 155七、交感神经节与脊神经节细胞突起制作方法 156第9 章 眼和耳 163一、眼球 163二、内耳 169第10 章 循环系统 175一、心脏 175二、动脉 176三、静脉 176四、心脏、大动脉、大静脉及中等动静脉的取材、固定与染色法的选择 176五、循环系统的染色方法 178六、血管注射 183第11 章 皮肤 187一、非角质形成细胞 187二、制作皮肤与其附属器的实验性指南 188三、皮肤标本的制作方法 189第12 章 免疫系统 196一、淋巴器官 196二、免疫系统的染色方法 198第13 章 内分泌系统 203一、内分泌腺 203二、内分泌腺的制作方法 206第14 章 消化管 226一、口腔与咽 226二、食管 227三、胃 228四、小肠与大肠 2314医学形态学染色技术理论与应用五、小肠上皮细胞 232六、肠管的神经 244第15 章 消化腺 251一、大唾液腺 251二、胰腺 252三、肝 264第16 章 呼吸系统 272一、嗅黏膜 272二、喉 272三、气管 272四、肺 273五、呼吸系统的切片标本制作方法 274第17 章 泌尿系统 279一、肾 279二、球旁复合体 280三、膀胱 280四、泌尿系统的染色方法 281笫18 章 男性生殖系统 285一、睾丸 285二、生殖管道 286三、附属腺 286四、阴茎 287五、男性生殖器官的取材、固定和染色 287六、男性生殖器官的染色方法 288第19 章 女性生殖系统 290一、卵巢 290二、子宫 291三、输卵管 291四、阴道 2925目录五、女性生殖器官的取材、固定与染色法的选择 292六、女性生殖系统的染色方法 293第20 章 胚胎 297一、胚胎的起源与发展 297二、胚胎标本的制作方法 298附录 常用名词术语中英文对照 304参考文献 311彩图 312
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